Macro photography is extreme close up photography, usually of very small subjects, in which the size of the subject in the photograph is greater than life size. By some definitions, a macro photograph is one in which the size of the subject on the negative or image sensor is life size or greater.However in other uses it refers to a finished photograph of a subject at greater than life size.
The ratio of the subject size on the film plane (or sensor plane) to the actual subject size is known as the reproduction ratio. Likewise, a macro lens is classically a lens capable of reproduction ratio greater than 1:1. Here some of my experiment used Canon macro lens 100 mm f/2.8L and flash diffuser.